-N-Acetylgalactosamine-capping of chondroitin sulfate core region oligosaccharides primed on xylosides

Regulation of Chondroitin Sulfate Synthesis EFFECT OF P-XYLOSIDES ON SYNTHESIS OF CHONDROITIN SULFATE PROTEOGLYCAN, CHONDROITIN SULFATE CHAINS, AND CORE PROTEIN*

Monolayer cultures of embryonic chick chondrocytes were incubated with %04*in the presence and absence of 1.0 mM p-nitrophenyl-/3-o-xyloside for 2 days. The relative amounts of chondroitin sulfate proteoglycan and free polysaccharide chains were measured following gel filtration on Sephadex G-200. Synthesis of P-xyloside-initiated polysaccharide chains was accompanied by an apparent decrease in...

Regulation of chondroitin sulfate synthesis. Effect of beta-xylosides on synthesis of chondroitin sulfate proteoglycan, chondroitin sulfate chains, and core protein.

Monolayer cultures of embryonic chick chondrocytes were incubated with 35SO42- in the presence and absence of 1.0 mM p-nitrophenyl-beta-d-xyloside for 2 days. The relative amounts of chondroitin sulfate proteoglycan and free polysaccharide chains were measured following gel filtration on Sephadex G-200. Synthesis of beta-xyloside-initiated polysaccharide chains was accompanied by an apparent de...

Synthesis of chondroitin sulfate oligosaccharides using N-tetrachlorophthaloyl and N-trifluoroacetyl galactosamine building blocks

Expression of N-Acetylgalactosamine 4-Sulfate 6-O-Sulfotransferase Involved in Chondroitin Sulfate Synthesis Is Responsible for Pulmonary Metastasis

Chondroitin sulfate (CS) containing E-disaccharide units, glucuronic acid-N-acetylgalactosamine(4, 6-O-disulfate), at surfaces of tumor cells plays a key role in tumor metastasis. However, the molecular mechanism of the metastasis involving the CS chain-containing E-units is not fully understood. In this study, to clarify the role of E-units in the metastasis and to search for potential molecul...

Stimulation of Synthesis of Free Chondroitin Sulfate Chains by p-D-Xylosides in Cultured Cells*

,&Xylosides stimulate 2to 6-fold the synthesis of glycosaminoglycans by three types of nonconnective tissue cells (RG-C6, NB41A, and rat hepatoma cells, and normal and simian virus 40 (SV40)-transformed normal human skin fibroblasts. The effect, which is specific for the anomeric linkage and the glycone, is observed in the presence and absence of puromycin. /3-Xylosides may substitute for xylos...